Sammuel:
I can tell that you have put a lot of thought and practice into this. I'd like to give you some feedback on your performance and your thinking.
First off, you seem to be over-proving a lot of things including that there are only two jokers at the beginning and that the queen is a single card and that there are only two jokers when you take them out of the pocket. From what I suspect is the method, you are proving things that really aren't an issue. I think part of that is that you are viewing the effect as a magician and wanting to stump your fellow magicians. However, the problem with doing that for lay audiences is that is just makes them more suspicious.
Second, your turnovers of the jokers and the queen (including turning the queen face down) seem unnatural. I know a lot of people give advice to make your single turnovers look like double turnovers, but that is assuming the single turnovers are being done from the top of the deck, not from the middle of the deck.
The cuts you use to lose the card in the deck are not fluid enough and, in my opinion, are too much. The audience is thinking he must be doing something. I would go for something more simple here such as a diagonal insertion or a double undercut. Probably the best sleight to use is Lee Asher's Losing Control followed by an overhand shuffle -- that way it looks like the card was inserted into the middle of the deck and then shuffled.
I do like how you got the queen into position for the final reveal, but the problem is doing the reveal on the top of the deck where you move both jokers above the queen. As an initial matter, you are handling the deck too much by even squaring it up -- anything you do at this point looks suspicious because the heat is on you. Further, the reveal doesn't work. If the card was between the jokers you would reveal it between the jokers. I like the thinking on the move behind the final reveal, but I think the move doesn't work in that situation and seems to have bad angles.
Now some advice about the thinking. How about this for an effect: Put two jokers in your jacket pocket, have the spectator pick a card and put it into the deck. Hand the deck to the spectator. Waive the jokers over the deck and show that a card has appeared between the jokers, reveal that it is the spectators card. Can be done with some set-up and single slight. It would look more magical to your audience. Sometimes simple is better.